Differential effects of oligosaccharides on the effectiveness of ampicillin against Escherichia coli in vitro

• AOS and GOS decreased the E. coli growth by different concentrations. AOS-Ampicillin exerted 2-fold decrease in MIC level of AMP against . reduced adherence to HT-29 cells. The combination further increased AOS’ anti-adhesive properties. AOS, COS or significantly hampered -induced IL-8 release HT-29. mounting antibiotic resistance emphasizes an urgent need for alternatives. Recent investigations indicate that non-digestible oligosaccharides (NDOs), besides their prebiotic properties, can directly interact with pathogenic bacteria. In this study, protective effect alginate-oligosaccharides (AOS), chitosan-oligosaccharides (COS), galacto-oligosaccharides (GOS) fructo-oligosaccharides, enteropathogenic Escherichia. was investigated. these NDOs on growth, adhesion inflammatory response (IL-8 release) intestinal epithelial cells were determined vitro presence absence ampicillin, using minimum inhibitory concentration (MIC) assay, anti-adhesion assay ELISA, respectively. At low concentrations 0.5 % 1%, while high (6%, 8%, 10 %) effective. Interestingly, ampicillin (2 μg/mL) a also dependently Pre-incubation release. Current study highlights direct effects responses